Nucleic acid mass spectrometry detection service-Wuhan Biobank Co., Ltd.

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Nucleic acid mass spectrometry detection service

Product Description

Case Analysis

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MassARRAY is based on Matrix Assisted Laser Desorption /Ionization-Time of Flight (MALDI-TOF) technology, and is the global original nucleic acid mass spectrometry platform for high precision DNA qualitative analysis. This technology platform

Perfectly integrates the high sensitivity of PCR technology, the high throughput of chip technology,the high accuracy of mass Spectrometry technology and the powerful function of computer Intelligent analysis, which is the best tool for large sample size Verification of a large number of candidate SNP and DNA methylation markers discovered by sequencing and chip technology.The MassARRAY system provides a unique solution for targeted gene detection using limited samples. It can meet the assay design, validation and performance needs of genomics laboratories.

Technology Principle

The MassARRAY time-of-flight mass spectrometry detection system consists of a matrix-assisted laser desorption ionization (MALDI) source, a time-of-flight mass analyzer (TOF), and a template preparation plate.The principle of MALDI is to irradiate a co-crystalline film formed by the sample and matrix with a laser, and the matrix absorbs energy from the laser and transmits it to the biomolecules, causing them to ionize.The principle of TOF is Biomolecules are ionized and accelerated through the flight tube by an electric field, and the mass-to-charge ratio of the ions is proportional to the ion's time of flight.

The system is extremely accurate and fast, allowing multiple samples to be tested at one time. Multiplex PCR amplification is first performed, followed by single-base extension with modified ddNTP, enabling the mass spectrometry system to accurately identify differences in individual bases and report the frequency of each base according to peak area, and thus accurately analyze the frequency of allele distribution in the sample.

Application scope

Detection of variant types :.

a.SNPs/SNVs (point mutations)

b.Indels (insertion/deletion mutations)

c.CNVs (copy number variants)

d.Gene Fusions

e.Methylations

Main application areas

a.Suitable for genome-wide SNP association studies and subsequent large sample size validation

b. Susceptibility gene analysis and gene localization for complex diseases

c.High sensitivity analysis of somatic mutations

d. Population genetics studies

e. Pharmacogenomics (drug development and individual drug use)

f. Other: pathogenic microbial detection, health management, biological sample identification, etc.

Available tests

Skin type genetic test	Advanced tumor susceptibility gene test for men(six items)
Metabolic genetic test	Advanced tumor susceptibility gene test for women (seven tests)
Weight and health management gene test	Deafness gene test
Scientific exercise gene test	Folic acid gene test
Breast cancer susceptibility gene test	Gene test for hypertension
Basic five tumor susceptibility gene test	Epilepsy gene test
Cardiovascular disease gene test

Project advantages

a. Multiplex reactions, fast detection cycle

Single reactions up to 10 to 60 assays and only simple PCR and extension reagents are required, no fluorescent probes are needed and the project lead time is within 5 working days.

b.High accuracy and sensitivity

Typing accuracy >99.7%, which is the gold standard for SNP detection; detection sensitivity up to 0.1% for low frequency alleles and rare mutations.

c.Flexible throughput

The standard 96 chips are available to meet the needs of different detection volumes, and the samples can be tested on the machine without making up samples.

d. High sample compatibility

Each reaction well requires only 5-10ng of genomic DNA, and supports biological samples from various sources: blood, blood collection cards, oral swabs, saliva, semen, fresh tissue, puncture tissue and FFPE samples, seeds, roots, leaves, etc. Even highly degraded samples can be tested.

e. Support different types of marker detection for a wide range of applications

It can detect SNP, point mutation, In/Del, CNV, gene fusion and methylation and many other variant types, which can be applied to genetic diseases, solid tumors and liquid biopsies, pharmacogenomics and many other fields.