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Multi-color pathology data analysis
Product description
Using spectral signal recognition technology, the overlapping multi-color signals could be accurately split, and with inForm intelligent quantitative pathology analysis software and professional bioinformatics analysis team, qualitative, conformal and quantitative analysis of tissues, cells and proteins in the images could be performed, and finally the data results are visually displayed in the form of visual figures, providing customers with standardized and customized data analysis to meet different types of needs.
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1. Scan the original image
2. Spectral resolution diagram
3. Organization and zoning map
4. Cell typing
5. Presentation data
6. Spatial analysis
Notices of samples delivery
1. Sample information
(1) Tissue origin: All specimens to be tested should provide accurate information about tissue type (e.g. melanoma, non-small cell lung cancer, colon cancer) and species (e.g. human origin, mouse).
(2) Labeling number: Each slide or wax block should be labeled with a clearly identifiable ‘unique’ number, and TMA slides should be provided with the chip layout and number.
2. Sample types
(1) Formalin-fixed wax blocks or slides, large pieces of tissue or TMA are acceptable.
(2) The sealing wax should not have obvious breakage.
(3) For slide samples, the tissue needs to fit snugly on the slide, avoiding folds, and the slide should not be broken, scratched or stained.
(4) The tissue should contain a minimum of more than 1000 cells.
3. Wax block requirements
(1) The wax block should be embedded with solid tumor tissue; necrotic tumor tissue, needle punctures tissue, and cytospin samples are not acceptable.
(2) Tissue should be fixed with 10% neutral formalin or 4% paraformaldehyde, with a fixation time of 18~24 hours.
(3) Samples fixed by other methods need to be clearly indicated.
(4) The wax block box should have clear and unambiguous number information (please do not just label with a simple number such as 1, 2, 3).
(5) Wax blocks should be stored in a sealed bag with the block number indicated.
4. Slides requirements
(1) The thickness of the slides should be about 3~4um. Please use anti-dislodging slides! (Very important)
(2) It is recommended that the slides should be prepared within one week after fixation.
(3) Prepare at least 2 slides for one sample to be tested.
(4) Do not add any adhesive during water bath.
(5) The sample should be placed in the middle of the slide.
(6) Place the slide upright on absorbent paper to remove water, remove water droplets by tapping slightly. Please do not wipe the slide with paper!
(7) Place the slide on a 45 degree hot plate for 30 min (naturally drying process should not be less than 1 hour).
(8) Slides must be completely dry before transportation.
(9) Each slide must be clearly numbered!
(10) Slides must be placed in a special slide box with the number and inatitution name clearly marked on the box.
(11) If slides have obvious folds and creases.
5. TMA Requirements
(1) Provide a description of the TMA chip point map (several rows and columns).
(2) The TMA slides must be matched with the chip point diagram.
(3) Minimum core point diameter >0.6mm.
(4) TMA core points must not overlap.
(5) Test slides derived from the same TMA wax block (usually incomplete slides from TMA sectioning for pre-experimental testing) need to be provided.
6. Transportation requirements
(1) Samples from different project groups can be transported together, but each group must be placed in a separate package and be clearly marked with: project number (customer order number) \ list of sample numbers \ contact person \ mobile phone number.
(2) Wax block: placed in a separate sealing pocket.
(3) Slides: placed inside the slide box, labeled clearly.
(4) TMA: placed inside the slide box with the instructions of the slide number.
(5) Please use an efficient courier channel such as S.F. to avoid samples being trasnported for a long time.
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