Instructions for single cell sequencing and sample delivery of Wuhan Biological Sample Bank
Single Cell ATAC
Product Description
Case Analysis
Result display
Sample delivery
Q&A
Single-cell ATAC-seq is to study chromatin accessibility at the single-cell level and is mainly used for the detection of transcriptional regulatory sequences, which can be combined with single-cell RNA-seq to analyse all actively transcribed regulatory sequences in the genome. This technique has been used in many research areas such as tumor heterogeneity studies, gene regulatory network analysis, cell lineage tracing, biomarker discovery, etc.
ATAC-Seq (Assay for Transposase Accessible Chromatin with high-throughput sequencing) uses Tn5 transposase to cleave nuclear chromatin regions which is open at a specific space-time and then adds primers for high-throughput sequencing to obtain the regulatory sequences of all active transcripts in the genome at that specific space-time condition.
ATAC-Seq schematic diagram
The single cell ATAC-Seq technology relies on the microfluidic technology of the 10x Genomics platform, combining characteristics of different cells of sequence tags distinguished population with ATAC-Seq technology to build chromatin accessibility profiles for thousands of single cells. Sample types: cell lines, primary cells, fresh and frozen tissues, etc.
Schematic diagram of 10x Genomics single-cell ATAC-Seq
Sample type
Cell lines, primary cells, fresh and frozen tissues, etc.
Experimental process
10x Genomics单细胞ATAC-Seq实验流程
Analysis process
Flow chart of single-cell ATAC analysis
Application directions
a. Studying cell heterogeneity;
b. Exploring biomarkers;
c. Defining cell types;
d. Building gene regulatory networks.
Product advantages
a. Detecting open chromatin in transcriptional regulatory regions of single cells;
b. Each channel can hold 500~10000 nuclei;
c. Nuclei capture rate up to 65%;
d. Suitable for the study of cell lines, primary cells, fresh and frozen samples;
e. Using the official 10x software, single cell gene regulation can be deeply analyzed.
2022-03-10 17:58:00
