Whole genome sequencing-Wuhan Biobank Co., Ltd.

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Whole genome sequencing

Product Description

Case Analysis

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Sample Submission Guidelines

Q&A

Whole Genome Sequencing (WGS) is a genome-wide sequencing method that can decipher all common and rare variants, single nucleotide polymorphisms (SNP), insertion and deletion sites (InDel), structural variants (SV) and copy number variants (CNV), and thus reveal all types of genomic mutations and chromosomal rearrangement events. This will provide guidance for disease mechanism research and drug target screening. Applications include clinical medicine, population genetics, association analysis, evolutionary analysis and so on.

technology roadmap

technical parameter

Technical Parameters

Sequencing strategy

Lead time

Sample type: Genomic DNA

Sample concentration: ≥20 ng/μl (Qubit quantification)

Total sample mass: ≥3.0 μg (total of 3 library builds, excluding consuming of sample assay, Qubit quantification)

Sample purity: OD260/280 =1.7~2.2

Electrophoresis requirements: clear primary bands, no degradation or light degradation, no serious RNA or protein contamination (latest electrophoresis results of the sample delivery date).

Sequencing mode: Illumina/MGI

Sequencing depth: 30X recommended

45 days

Product advantages

a. High accuracy: Using the Illumina /MGI platform by which more than a million sequences can be performed in a single run, with accurate counts ranging from several to hundreds of thousands copies;

b. Wide detection range: single nucleotide polymorphism (SNP), insertion deletion (InDel), structural variation (SV), copy number variation (CNV) and other variants can be detected, and new variant sequences can be detected compared to microarrays;

c. Low cost and less time consuming than whole human genome de novo sequencing.