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Single Cell Multiome ATAC + Gene Expression
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Single Cell Multiome ATAC + Gene Expression

Product Description
Case Analysis
Result display
Sample delivery
Q&A

Single Cell Multiome ATAC + Gene Expression combines ATAC with transcriptome sequencing to identify complex cell populations through integrated analysis of epigenomic and gene expression profiles and detect cellular heterogeneity to uncover hidden information. Combining the discovery of regulatory elements with gene expression could help to explore the gene regulatory interactions that drive cell differentiation, development and disease.

Sample types: animal tissues, single cell suspensions, nuclear suspensions, cell lines.

 

Experimental process

Flow Chart of ATAC+Gene Expression Experiment in Single Cell Multiomics

Analysis process

Flow Chart of ATAC+Gene Expression Analysis in Single Cell Multiomics

  

Application directions

a. Biomarker discovery;

b. Cell lineage and developmental program tracking;

c. Cell heterogeneity and detection of rare cell populations;

d. Gene regulatory networks;

e. Response to therapeutic interventions.

 

Product advantages

a. Efficiently distributing 500-10,000 nuclei per lane, analyzing up to 80,000 nuclei per run ;

b. Extensible to run up to 8 samples in parallel;

c. Nuclei recovery up to 65%;

d. High sensitivity;

e. Low microfluidic double rate (less than 1% per 1,000 nuclei);

f. Suitable for studies with cell lines, primary cells, fresh and frozen samples.

Case 1 Mechanistic exploration of a subtype of a new spectrum of blurred acute leukaemia: enhancer hijacking of BCL11B

Leukaemias of unknown spectrum is a kind of high-risk malignancies whose genetic basis is not clear. Investigators describe a distinct subpopulation of acute leukaemias that express myeloid, T-lymphocyte and stem cell markers activated by aberrant allele-specific dysregulation of BCL11B, a major transcription factor in the thymic T system. Mechanistically, this dysregulation is caused by chromosomal rearrangements that juxtapose BCL11B with hyperenhancers active in haematopoietic progenitor cells or amplify the generation of hyperenhancers from non-coding elements distal to BCL11B. Chromatin conformational analysis revealed a prolonged interaction between the rearranged enhancers and the expression of the BCL11B allele, and an association between BCL11B and activated haematopoietic progenitor cell cis-regulatory elements. This suggests that BCL11B is aberrantly incorporated into a gene regulatory network that drives transformation by maintaining the progenitor cell state. These data suggest a role for enhancer hijacking-mediated ectopic BCL11B expression in primitive haematopoietic cells as an oncogenic driver of human spectrum-unknown leukaemias.

1

 

Reference:Montefiori L E , Bendig S , Gu Z , et al. Enhancer hijacking drives oncogenic BCL11B expression in lineage ambiguous stem cell leukemia[J]. Cancer Discovery, 2021:candisc.0145.2021.

Single cell atlas

 

Differential genes between clusters

Differences between clusters peak

Identification of putative regulatory elements directly associated with target genes

Motif enrichment

 

Instructions for single cell sequencing and sample delivery of Wuhan Biological Sample Bank

     

Q1:What analysis can be carried out with single cell multi-omics ATAC+ gene expression?

A: It enables simultaneous analysis of the transcriptome and epigenome (using ATAC-seq) of single cells to help you understand how genes are expressed and regulated between different cell types.

Q2:What's the sensitivity of single-cell multi-omics ATAC+ gene expression compared to single-cell transcriptome and single-cell ATAC analysis alone?

A: Among the sample types which could be tested by Single Cell Multicomics ATAC+ Gene Expression, the performance of ATAC and gene expression is comparable to the corresponding 10x Genomics processes alone (Chromium Single Cell ATAC or Chromium Single Cell Gene Expression) when the starting sample is the nucleus.

Q3:Is single cell multi-omics ATAC + gene expression compatible with Feature Barcode technology?

A: At present, Feature Barcode technology is not compatible with Single Cell Multi-omics ATAC + Gene Expression.

Q4:What types of samples can be used?

A: Cell nuclear suspensions is required. High quality nuclei suspensions can be obtained from fresh and frozen cells, fresh tissue and frozen tissue. This analysis has been optimised for human and mouse samples.

Q5:Can data from single cell multi-omics ATAC+ gene expression be combined with data from individual analyses?

A: Yes, the file formats for single cell ATAC and single cell gene expression data are compatible with single cell multi-omics ATAC+ gene expression and can be integrated using third party tools such as Seurat.

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  • Instructions for single cell sequencing and sample delivery of Wuhan Biological Sample Bank

    Instructions for single cell sequencing and sample delivery of Wuhan Biological Sample Bank
    File size: 360KB 发布时间: 2022-03-10

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