Ready-to-use multiplex immunofluorescence panel_Wuhan biological Optical Valley Science and technology

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Ready-to-use multiplex immunofluorescence panel

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Ready-to-use multiplex immunofluorescence panel

Product Description

Case Analysis

Result display

Sample delivery

Q&A

Product introduction

This product is used for tumor immune microenvironment assessment and clinical development and translational research of tumor immune drugs, covering multiple dimensions such as tumor immunotherapy targets and tumor immune cells. Through high specificity multiplex fluorescence staining, spectral imaging and high throughput image analysis technologies, we can accurately capture tumor microenvironment data to empower anti-tumor drug development.

Sample image of product BB02

Service products and cycle

Product Number	Indicators	Tissue Phenotype	Data Analysis	Lead time
BB01	CD3、CD20、CD68、 CD163、Ki67、pan-CK、DAPI	T cells, B cells, M1 macrophages, M2 macrophages	Standard analysis. 1. tissue partitioning: tumor and interstitial. 2. cell typing: defining the different cell types separately and calculating the number, density and percentage of cells. Extended analysis. 1. Evaluation of expression indicators TPS and CPS; 2 statistics on the number, density, and percentage of immune checkpoint molecules expressed in different types of cells. 3. spatial analysis.	10 working days
BB02	CD4、CD8、FOXP3、 PD-1、PD-L1、pan-CK、DAPI	Helper T cells, killer T cells
BB03	CD8、CD68、CD56、 PD-1、PD-L1、pan-CK、DAPI	Treg, immune checkpoint killer T cells, total macrophages, Depleted T cells, NK cells, immune checkpoints
BB04	PD-1、PD-L1、CD68、 CD8、CD163、pan-CK、DAPI	M1 macrophages, M2 macrophages, killer T cells, immune checkpoints
BB05	CD3、CD4、CD20、 CD56、FOXP3、pan-CK、DAPI	depleted T cells, immune checkpoints total T cells, helper T cells. B cells, NK cells, Treg
BB06	CD4、CD8、GZMB、 Ki67、FOXP3、pan-CK、DAPI	depleted T cells, immune checkpoints total T cells, helper T cells. B cells, NK cells, Treg
BB07	CD11c、CD20、CD68、 CD163、CD3、pan-CK、DAPI	tertiary lymphoid structures, B cells, T cells, and Germinal center activated B cells, follicular dendritic cells Tertiary lymphoid structures, B cells, killer T cells
BB08	CD20、CD21、CD23、 CD3、Ki67、pan-CK、DAPI	tertiary lymphoid structures, B cells, T cells, and Germinal center activated B cells, follicular dendritic cells
BB09	CD20、CD23、CD8、 PD-1、PD-L1、pan-CK、DAPI	Tertiary lymphoid structures, B cells, killer T cells follicular dendritic cells, depleted T cells, immune checkpoints
BB10	PD-1、CD8、CD68、 CD56、FOXP3、pan-CK、DAPI	killer T cells, total macrophages, NK cells, Treg, immune checkpoints

Notices of samples delivery

1. Sample information

(1) Tissue origin: All specimens to be tested should provide accurate information about tissue type (e.g. melanoma, non-small cell lung cancer, colon cancer) and species (e.g. human origin, mouse).

(2) Labeling number: Each slide or wax block should be labeled with a clearly identifiable unique number, and TMA slides should be provided with the chip layout and number.

2. Sample types

(1) Formalin-fixed wax blocks or slides, large pieces of tissue or TMA are acceptable.

(2) The sealing wax should not have obvious breakage.

(3) For slide samples, the tissue needs to fit snugly on the slide, avoiding folds, and the slide should not be broken, scratched or stained.

(4) The tissue should contain a minimum of more than 1000 cells.

3. Wax block requirements

(1) The wax block should be embedded with solid tumor tissue; necrotic tumor tissue, needle punctures tissue, and cytospin samples are not acceptable.

(2) Tissue should be fixed with 10% neutral formalin or 4% paraformaldehyde, with a fixation time of 18~24 hours.

(3) Samples fixed by other methods need to be clearly indicated.

(4) The wax block box should have clear and unambiguous number information (please do not just label with a simple number such as 1, 2, 3).

(5) Wax blocks should be stored in a sealed bag with the block number indicated.

4. Slides requirements

(1) The thickness of the slides should be about 3~4um. Please use anti-dislodging slides! (Very important)

(2) It is recommended that the slides should be prepared within one week after fixation.

(3) Prepare at least 2 slides for one sample to be tested.

(4) Do not add any adhesive during water bath.

(5) The sample should be placed in the middle of the slide.

(6) Place the slide upright on absorbent paper to remove water, remove water droplets by tapping slightly. Please do not wipe the slide with paper!

(7) Place the slide on a 45 degree hot plate for 30 min (naturally drying process should not be less than 1 hour).

(8) Slides must be completely dry before transportation.

(9) Each slide must be clearly numbered!

(10) Slides must be placed in a special slide box with the number and inatitution name clearly marked on the box.

(11) If slides have obvious folds and creases.

5. TMA Requirements

(1) Provide a description of the TMA chip point map (several rows and columns).

(2) The TMA slides must be matched with the chip point diagram.

(3) Minimum core point diameter >0.6mm.

(4) TMA core points must not overlap.

(5) Test slides derived from the same TMA wax block (usually incomplete slides from TMA sectioning for pre-experimental testing) need to be provided.

6. Transportation requirements

(1) Samples from different project groups can be transported together, but each group must be placed in a separate package and be clearly marked with: project number (customer order number) \ list of sample numbers \ contact person \ mobile phone number.

(2) Wax block: placed in a separate sealing pocket.

(3) Slides: placed inside the slide box, labeled clearly.

(4) TMA: placed inside the slide box with the instructions of the slide number.

(5) Please use an efficient courier channel such as S.F. to avoid samples being trasnported for a long time.