Protein qualitative analysis services-Wuhan Biobank Co., Ltd.

登录注册 EN

Home page

科技服务

Single Cell Sequencing Service
- Single Cell Sequencing
- Spatial transcriptome sequencing
  - Spatial Gene Expression for Fresh Frozen
  - Spatial Gene Expression for FFPE
High throughput sequencing services
Cell-based immune-related assays
Proteomics Testing Services
- Proteomics services by LC/MS
- Olink Proteomics Services
  - Olink Precision Proteomics Analysis Service
Small molecule metabolite detection services
- Metabolomics Services
  - Targeted metabolomics
  - Non-targeted metabolomics
- Nucleic acid mass spectrometry detection service

Protein qualitative analysis services

Product Description

Case Analysis

Results Display

Sample Submission Guidelines

Q&A

a. Protein full-spectrum analysis

Protein full-spectrum analysis can also be called mass spectrometry shotgun analysis. which refers to the component analysis.The Objects of study are complete tissues, body fluids or their extracts, with the aim of identifying as many peptides and protein molecules as possible. The basic principle of protein mass spectrometry identification is to digest proteins into peptide mixtures by proteases, and then ionize them by electron bombardment or other means to form charged ions with different mass-to-charge ratios, and then separate the peptide ions with specific mass-to-charge ratios by a mass analyzer. The protein was identified by comparing the actual spectrogram with the primary and secondary mass spectra of protein digested by protease in theory.

Schematic diagram of protein full spectrum analysis

Instruments used: Orbitrap Eclipse, Orbitrap Exploris 480, Q Exactive plus; AB SCIEX: TripleTOF 5600 plus.

Technical advantages: Comprehensive identification of a large number of proteins in whole cells, tissues or complex mixed samples, with 200-5000 proteins can be identified.

Application areas: Identification of all expressed proteins in tissues, cells or organelles in a given physiological state.

Sample requirements:

Sample	Sample requirements
Protein solution	Total protein > 500 μg, concentration > 0.1 μg/μL; Buffer solution without detergent NP40, Triton X-100, etc.

Lead time（Project Cycle）

Progress	Time period
Sample collection/Quality control	2-3 working days
Sample processing	4-5 working days
Mass spectrometry detection	2-3 working days
Data analysis	2-3 working days
Total	No more than 15 working days

Note: For more than 5 samples, 3 working days will be added for each additional sample. If there is an urgent need, please contact us for negotiation.

b. Protein Identification

LC-MS/MS technology was used to identify proteins for moderately complex samples such as single strips (i.e., SDS-PAGE samples), IP, Co-IP, and Pull-down purification solutions.

凝胶电泳条带鉴定路线图

Pull-down experiment roadmap

Instruments: Orbitrap Eclipse, Orbitrap Exploris 480, Q Exactive Plus .

Technical advantages: high sensitivity, high accuracy. Suitable for moderately complex protein samples, such as IP, Co-IP .

Application areas: disease marker research, mechanism of action research, plant resistance research, drug target research, specific functional protein identification.

Sample requirements:

Sample	Sample requirements
SDS-PAGE bands	Komas staining, silver staining (mass spectrometry compatible) bands are clearly visible
Protein solution	Total protein >5μg, concentration >0.1μg/μL;
Protein solution	buffer without detergent NP40, Triton X-100, etc.

Lead time（Project Cycle）

Progress	Time period
Sample collection/Quality control	1-2 working days
Sample processing	2-3 working days
Mass spectrometry detection	2-3 working days
Data analysis	2-3 working days
Report generation	1-2 working days
Total	No more than 10 working days

Note: For more than 10 samples, 1 working days will be added for each additional sample. If there is an urgent need, please contact us for negotiation.