Protein qualitative analysis services
a. Protein full-spectrum analysis
Protein full-spectrum analysis can also be called mass spectrometry shotgun analysis. which refers to the component analysis.The Objects of study are complete tissues, body fluids or their extracts, with the aim of identifying as many peptides and protein molecules as possible. The basic principle of protein mass spectrometry identification is to digest proteins into peptide mixtures by proteases, and then ionize them by electron bombardment or other means to form charged ions with different mass-to-charge ratios, and then separate the peptide ions with specific mass-to-charge ratios by a mass analyzer. The protein was identified by comparing the actual spectrogram with the primary and secondary mass spectra of protein digested by protease in theory.
Schematic diagram of protein full spectrum analysis
Instruments used: Orbitrap Eclipse, Orbitrap Exploris 480, Q Exactive plus; AB SCIEX: TripleTOF 5600 plus.
Technical advantages: Comprehensive identification of a large number of proteins in whole cells, tissues or complex mixed samples, with 200-5000 proteins can be identified.
Application areas: Identification of all expressed proteins in tissues, cells or organelles in a given physiological state.
Sample requirements:
Sample |
Sample requirements |
Protein solution |
Total protein > 500 μg, concentration > 0.1 μg/μL; Buffer solution without detergent NP40, Triton X-100, etc. |
Lead time(Project Cycle)
Progress |
Time period |
Sample collection/Quality control |
2-3 working days |
Sample processing |
4-5 working days |
Mass spectrometry detection |
2-3 working days |
Data analysis |
2-3 working days |
Total |
No more than 15 working days |
Note: For more than 5 samples, 3 working days will be added for each additional sample. If there is an urgent need, please contact us for negotiation.
b. Protein Identification
LC-MS/MS technology was used to identify proteins for moderately complex samples such as single strips (i.e., SDS-PAGE samples), IP, Co-IP, and Pull-down purification solutions.
凝胶电泳条带鉴定路线图
Pull-down experiment roadmap
Instruments: Orbitrap Eclipse, Orbitrap Exploris 480, Q Exactive Plus .
Technical advantages: high sensitivity, high accuracy. Suitable for moderately complex protein samples, such as IP, Co-IP .
Application areas: disease marker research, mechanism of action research, plant resistance research, drug target research, specific functional protein identification.
Sample requirements:
Sample |
Sample requirements |
SDS-PAGE bands |
Komas staining, silver staining (mass spectrometry compatible) bands are clearly visible |
Protein solution | Total protein >5μg, concentration >0.1μg/μL; |
buffer without detergent NP40, Triton X-100, etc. |
Lead time(Project Cycle)
Progress | Time period |
Sample collection/Quality control |
1-2 working days |
Sample processing |
2-3 working days |
Mass spectrometry detection |
2-3 working days |
Data analysis |
2-3 working days |
Report generation |
1-2 working days |
Total |
No more than 10 working days |
Note: For more than 10 samples, 1 working days will be added for each additional sample. If there is an urgent need, please contact us for negotiation.