Flow cytometry special service
(1) Identification of Extracellular Vesicles (Flow cytometry + Single cell sequencing)
Extracellular vesicles (EV) are considered as potential non-invasive biomarkers in disease diagnosis and mediators in drug delivery with a high degree of heterogeneity. Transcriptome studies of single EVs are valuable to better define EV characteristics, EV subgroups and clinical applications. For EV identification and concentration assessment, it is a critical step for single cell sequencing to be successfully brought online. The use of flow cytometry-based means enables the size-specific localization of EVs, thus allowing the enumeration of integrity vesicles as well as the adjustment of appropriate concentrations for subsequent single-cell sequencing.
(2) Study of tumor immune microenvironment (Flow cytometry + single cell sequencing + spatial transcriptome + multicolor immunofluorescence)
The tumor microenvironment is a complex environment for tumor cells to survive and thrive. Tumor cells can evade the attack of immune cells, and grow rapidly and metastasize, leading to easy recurrence of the disease and poor prognosis. There are many studies on immunity in the tumor microenvironment, such as studying the immune activity of T cells and B cells at the cellular level. There are also a large number of studies combining spatial location, such as the finding that an increase in TIL-B and tertiary lymphoid structures (TLS) in germinal centers correlates with a good prognosis of tumor treatment.
(3) Plant genome size assay (flow cytometry + sequencing)
Genome size or C-value refers to the total amount of DNA contained in the eukaryotic haploid genome, which is species-specific and stable, and is a fundamental parameter of biological genome diversity. Flow cytometry assay is a relatively fast and effective method to identify the size of plant genomes. The assay can be followed by accurate evaluation in our sequencing platform.
Project advantages.
Wuhan Biobank's multi-omics platform has undergone 10 years of technological innovation. Led by several PhDs, the team has accumulated a lot of project experience. The multi-omics platform specifically includes three major segments: Cell Department, Sequencing Department and Protein Department , equipped with medium and large scale equipment such as flow cytometry, multicolor immunofluorescence, high throughput sequencing, single cell sequencing, spatial transcriptomics, mass spectrometry, NMR and Olink proteome. The platform can achieve multi-omics joint research of genome, epigenome, transcriptome, proteome, metabolome, etc.
At present, the platform has processed over hundreds of types of tissue samples and developed various tissue dissociation methods for different types of samples to solve the problem of samples not being able to come online. The platform includes a professional biochemical analysis team, which can personalize the analysis strategy. The platform includes one-stop technical services and facilitates the acceptance and publication of papers. The platform has helped many clinical scientists to publish many high-level scientific papers in top journals.
Project cycle: overall project, one project one negotiation
