Immune cell typing and functional assessment-Wuhan Biobank Co., Ltd.

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Immune cell typing and functional assessment

Product Description

Case Analysis

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Sample Submission Guidelines

Q&A

Flow cytometry (FCM) is a multi-parameter, rapid quantitative analysis and sorting technique for cells or particles in a fast, linear and stable flow state using a laser as the light source. In addition to conventional apoptosis and cycling, it can be applied to immune cell typing, intracellular/extracellular cytokine and phosphorylated protein detection, stem cell phenotype identification, plant ploidy/genome size assessment, and high-throughput single cell sorting for downstream sequencing.

With the development of immunology research, the immune indicators detected are studied deeper and with more diversity. Based on the multi-indicator high-throughput detection feature of flow cytometry, multiple indicators such as T/B/NK, monocyte, macrophage, Th1/Th2/Th17 can be detected simultaneously for classification of immune cells and functional assessment of intracellular factor expression.

Instrument used: Cytoflex S (4 laser 13 colors), Cytoflex LX (6 laser 21 colors)

Application areas: immunology, hematology, oncology, bone marrow and organ transplantation, antitumor drug research, vaccine research, basic medical research, etc.

Project advantages.

Highly configurable flow cytometry analysis/sorting instrument; Clinical/scientific research sampling directly to the laboratory; After completing tissue dissociation, flow cytometry analysis/sorting can be matched to our sequencing, single cell sequencing, proteomics, biochemical analysis and other platforms one-stop service with no middlemen to earn the difference.

Professional technicians with decades of experience in flow cytometry assay and cellular experiments could provide professional technical guidance; The team have supported the analysis of more than 100,000 samples and have accumulated rich experience in developing research projects, and could provide targeted and high-quality customized services.

Lead time:

Project Type	Project Content	Service Period
Raw cell material acquisition	Whole blood PBMC isolation	1-3 working days
Raw cell material acquisition	Preparation of single cell suspension from tissue	1-3 working days
Immunocytotyping	T/B/NK	1-3 working days
Immunocytotyping	Monocytes, macrophages, granulocytes, DC, MDSC, etc.	1-3 working days
Altered immune cell differentiation and activation ratio	Treg/Th1/Th2/Th9/Th17/Th22/Memory T/Tfh/Memory B, GCB/Macrophage M1/M2, etc.	1-3 working days
Functional assessment of intracellular factor expression	Intracellular factors such as Th1/Th2/Th9/Th17/Th22 1-3 working days	1-3 working days

Note: The above lead time is under the condition of reagents provided by Party A. If Party B's existing reagents have been exhausted, the procurement time of 4-5 weeks would be included.