Immune cell typing and functional assessment_Wuhan biological Optical Valley Science and technology

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Immune cell typing and functional assessment

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Immune cell typing and functional assessment

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Flow cytometry (FCM) is a multi-parameter, rapid quantitative analysis and sorting technique for cells or particles in a fast, linear and stable flow state using a laser as the light source. In addition to conventional apoptosis and cycling, it can be applied to immune cell typing, intracellular/extracellular cytokine and phosphorylated protein detection, stem cell phenotype identification, plant ploidy/genome size assessment, and high-throughput single cell sorting for downstream sequencing.

With the development of immunology research, the immune indicators detected are studied deeper and with more diversity. Based on the multi-indicator high-throughput detection feature of flow cytometry, multiple indicators such as T/B/NK, monocyte, macrophage, Th1/Th2/Th17 can be detected simultaneously for classification of immune cells and functional assessment of intracellular factor expression.

Instrument used: Cytoflex S (4 laser 13 colors), Cytoflex LX (6 laser 21 colors)

Application areas: immunology, hematology, oncology, bone marrow and organ transplantation, antitumor drug research, vaccine research, basic medical research, etc.

Project advantages.

Highly configurable flow cytometry analysis/sorting instrument; Clinical/scientific research sampling directly to the laboratory; After completing tissue dissociation, flow cytometry analysis/sorting can be matched to our sequencing, single cell sequencing, proteomics, biochemical analysis and other platforms one-stop service with no middlemen to earn the difference.

Professional technicians with decades of experience in flow cytometry assay and cellular experiments could provide professional technical guidance; The team have supported the analysis of more than 100,000 samples and have accumulated rich experience in developing research projects, and could provide targeted and high-quality customized services.

Lead time:

Project Type	Project Content	Service Period
Raw cell material acquisition	Whole blood PBMC isolation	1-3 working days
Raw cell material acquisition	Preparation of single cell suspension from tissue	1-3 working days
Immunocytotyping	T/B/NK	1-3 working days
Immunocytotyping	Monocytes, macrophages, granulocytes, DC, MDSC, etc.	1-3 working days
Altered immune cell differentiation and activation ratio	Treg/Th1/Th2/Th9/Th17/Th22/Memory T/Tfh/Memory B, GCB/Macrophage M1/M2, etc.	1-3 working days
Functional assessment of intracellular factor expression	Intracellular factors such as Th1/Th2/Th9/Th17/Th22 1-3 working days	1-3 working days

Note: The above lead time is under the condition of reagents provided by Party A. If Party B's existing reagents have been exhausted, the procurement time of 4-5 weeks would be included.

Sample Type		Sample Requirements	Notes
Cells	Cells in culture	Fill the culture bottle with cell culture solution, tighten the cap and seal with sealing film	Please keep 4℃ for in-city delivery (in case of ice bag, physical isolation is required to avoid freezing) and deliver to our laboratory within 2 hours; if mailing samples, only frozen cells can be mailed on dry ice
	Cell suspension	After digestion with trypsin into a single cell suspension (or suspension without trypsin) and washing with PBS, count live cells, make sure the viability is over 80%, and adjust the cell concentration to (5-10)×105 cells/mL.
	Cryopreserved cells	Cell concentration of (5-10) × 105 cells/mL, viability >80%	Dry ice delivery
Blood	Whole blood	Anticoagulated whole blood (volume needs to be communicated according to the specific type of test) to confirm that the blood is non-coagulated and non-hemolyzed	1. if intracellular cytokines need to be detected, it is recommended to use heparin anticoagulation tubes to store the anticoagulated blood and avoid using EDTA anticoagulation tubes. 2. avoid violent collisions and rapid temperature change during the transportation of blood samples, keep them at 4℃ (in case of ice bags, physical isolation is required to avoid freezing) and deliver them to our laboratory within 24h. 3. If the sample cannot be tested immediately and needs to be frozen, the whole blood sample cannot be frozen, and PBMC needs to be separated before freezing; the frozen sample needs to be transported on dry ice.
Blood	PBMC	Lysate or lymphatic isolate to obtain PBMC, freeze
Tissue samples (spleen, lymph nodes, lung, subcutaneous tumors, etc.)		Fresh tissue should be no less than 0.5 cm × 0.5 cm × 0.5cm in size, necrotic tissue needs to be removed and blood needs to be cleaned; preserve the sample with tissue preservation solution and keep the sample in a 4°C environment (in case of ice bags, it needs to be physically isolated to avoid tissue freezing) and send to our laboratory within 24h	1. fresh tissue samples are taken in situ and preserved with preservation solution as soon as possible within 1h. 2. If the sample cannot be tested within 48h, it needs to be frozen and stored, and the tissue should not be frozen directly, it needs to be processed into single cell suspension and then frozen, and transported on dry ice