Methylation sequencing
Genome methylation sequencing (Bisulfate Sequencing, BS-seq) refers to the treatment of whole genome DNA with heavy sulfite, which can convert unmethylated cytosine bases into uracil bases, and high-throughput sequencing to detect all methylation sites in the whole genome, and drawing DNA methylation map with single-base resolution. Genome-wide methylation sequencing can provide a comprehensive understanding of the mechanisms underlying epigenetic differences in embryonic development, aging, and disease, and provide guidance for research.
Technical route:
Technical parameters:
|
Sample requirements |
Sequencing strategy |
Lead time |
|
Sample type: DNA samples Sample concentration: ≥50ng/μl Total sample mass: ≥5μg |
Sequencing mode: Illumina/MGI PE150 Sequencing depth: 30-50X |
45 days |
Product advantages.
a. Library building starting mass as low as 50ng;
b. Good reproducibility of the coverage area of multiple samples, suitable for the analysis of differences between multiple samples;
c. Wide range of detection: cover the methylation status of every C base in the whole genome;
