搜索
Current location:
Homepage
/
/
/
/
Multiple cytokine expression assessment
技术平台

Multiple cytokine expression assessment

Product Description
Case Analysis
Result display
Sample delivery
Q&A

Cytokines, as intercellular signaling molecules, mainly mediate and regulate the immune response and inflammatory reaction, and maintain the stability of the body's immune system by regulating the dynamic balance between immune promotion and immune suppression. The detection of cytokines is of great value for the diagnosis and treatment of certain diseases. Extracellular (serum, cell culture fluid, lavage fluid, etc.) cytokines reflect the level of secretion.

 

 

Instrument used: Cytoflex S (4 laser 13 colors), Cytoflex LX (6 laser 21 colors)   

Application areas: immunology, hematology, oncology, bone marrow and organ transplantation, antitumor drug research, vaccine research, basic medical research, etc.

Project advantages

Flow cytometry extracellular factor assay (superior to Elisa, more than 10 cytokines simultaneously in 5 hours can be detected with only 25 μL sample, which greatly saves the amount of sample, time cost and experimental cost, and has high sensitivity and specificity)

Lead time:

Project type Project content Service cycle
Detection of secretory cytokine concentrations in body fluids Th1/Th2/Th9/Th17/Th22/anti-inflammatory, pro-inflammatory cytokines (IL-2, IL-6, IL-10, IFN-γ, TNF-α, IL-5, IL-13, IL-4, IL-17A, IL-17F, IL-22, IL-9, CXCL8 (IL-8), CXCL10 (IP-10), CCL11 (Eotaxin), CCL17 (TARC), CCL2 (MCP-1), CCL5 (RANTES), CCL3 (MIP-1α), CXCL9 (MIG), CXCL5 (ENA-78), CCL20 (MIP-3α), CXCL1 (GROα), CXCL11 (I-TAC), etc. )

1-3 working days

Note: The above lead time is under the condition of reagents provided by Party A( the client ). If Party B (the company )'s previous reagents have been ran out ,another 4-5 weeks is needed in addition for purches related reagents .

Signed article: Preclinical immunogenicity assessment of a cell-based inactivated whole-virion H5N1 influenza vaccine.

Journal: Open Life Sciences

Sample type: mouse serum

Technique used: Flow cytometry

Abstract: In this article, immune cell typing, quantification of cytokine expression and classification of immunoglobulin G (Ig G) subtypes were used to study the vaccine-induced immune response. The protective effect of the vaccine on mice was evaluated using an attack assay, Anti-H5N1 antibodies in rats persisted for 8 months after the first injection. Compared to the placebo group, serum titers of vaccinated mice were significantly higher, Th1 and Th2 cells were activated, and CD8+ T cells were activated in both groups. In addition, the attack assay showed that vaccination reduced clinical signs and viral titers in the lungs of mice after the attack, indicating a superior immune response. Notably, a significant increase in interferon-inducible protein-10 (IP-10) levels was found in the early post-vaccination period, indicating improved vaccine-induced innate immunity.

 

 

Assessment of Th1/Th2 cytokine levels after vaccine immunization  

 

Flow cytometry figure of 8 Th cytokines in mouse serum

 

 

Flow cytometry figure of 10 inflammatory factors in mouse serum

 

 

Flow cytometry figure of  9 pro-inflammatory factors in mouse serum

Sample Type Sample Requirements

Precautions

Blood

 

Whole blood     

Anticoagulated whole blood (volume to be communicated according to the specific type of test), confirmed to be free of coagulation and hemolysis

1. if intracellular cytokines need to be detected, the storage is recommended with heparin anticoagulation tubes but EDTA anticoagulation tubes.

2. avoid violent collisions and rapid temperature rise and fall during the transportation of blood samples, keep them at 4℃ (in case of ice bags, physical isolation is required to avoid freezing of blood samples) and deliver them to our laboratory within 24h.

3. If the sample cannot be tested immediately and need pretreatment, the whole blood sample cannot be frozen, and PBMC needs to be separated before freezing; the frozen sample needs to be transported on dry ice.

 

Plasma/Serum

Leave or centrifuge whole blood, take 200-1000 μL of supernatant in EP tube and freeze it with number. (-20℃ for one month, -80℃ for about 3 months)

Other fluids such as ascites, cerebrospinal fluid, cultured cell supernatant, etc.  After centrifugation, collect 200-1000 μL of supernatant in EP tubes and freeze them with numbers. (-20℃ for one month, -80℃ for about 3 months)

Please keep at 4℃ for delivery within the city and deliver to our laboratory within 2 hours; if mailing samples, only frozen samples can be mailed on dry ice

 

Follow us

Link: