Multiple cytokine expression assessment
Cytokines, as intercellular signaling molecules, mainly mediate and regulate the immune response and inflammatory reaction, and maintain the stability of the body's immune system by regulating the dynamic balance between immune promotion and immune suppression. The detection of cytokines is of great value for the diagnosis and treatment of certain diseases. Extracellular (serum, cell culture fluid, lavage fluid, etc.) cytokines reflect the level of secretion.
Instrument used: Cytoflex S (4 laser 13 colors), Cytoflex LX (6 laser 21 colors)
Application areas: immunology, hematology, oncology, bone marrow and organ transplantation, antitumor drug research, vaccine research, basic medical research, etc.
Project advantages
Flow cytometry extracellular factor assay (superior to Elisa, more than 10 cytokines simultaneously in 5 hours can be detected with only 25 μL sample, which greatly saves the amount of sample, time cost and experimental cost, and has high sensitivity and specificity)
Lead time:
Project type | Project content | Service cycle |
Detection of secretory cytokine concentrations in body fluids | Th1/Th2/Th9/Th17/Th22/anti-inflammatory, pro-inflammatory cytokines (IL-2, IL-6, IL-10, IFN-γ, TNF-α, IL-5, IL-13, IL-4, IL-17A, IL-17F, IL-22, IL-9, CXCL8 (IL-8), CXCL10 (IP-10), CCL11 (Eotaxin), CCL17 (TARC), CCL2 (MCP-1), CCL5 (RANTES), CCL3 (MIP-1α), CXCL9 (MIG), CXCL5 (ENA-78), CCL20 (MIP-3α), CXCL1 (GROα), CXCL11 (I-TAC), etc. ) |
1-3 working days |
Note: The above lead time is under the condition of reagents provided by Party A( the client ). If Party B (the company )'s previous reagents have been ran out ,another 4-5 weeks is needed in addition for purches related reagents .