Macro-transcriptome sequencing

Product Description

Case Analysis

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Sample Submission Guidelines

Q&A

Macro-transcriptomics studies the genome-wide transcription of organisms and the regulation of transcription at a holistic level for a specific environment and a specific period of time in a community. Macro-transcriptome sequencing can study in situ the composition of active strains and the expression of active genes in a microbial community of the specific habitat at a specific time and space. In combination with the detection of physicochemical factors, macro-transcriptome can study the differences in the composition of active components between microbial communities in space and time due to the differences in physicochemical and other indicators among the samples.

Technical route:

1

 

 

  Sample requirements:

Sample type

Sequencing strategy

Lead time

Sample concentration: ≥100 ng/μl

Total sample  mass: ≥4 μg

Sample purity: OD260/280=1.8~2.2, OD260/230=1.8~2.2; 28S: 18S≥1.

Integrity:RIN≥7

Sequencing mode: Illumina/MGI PE150

Sequencing depth: 6-12G clean data

45 days

 

Product advantages:
A. Technical advantages: the official ribosome removal kit can effectively remove rRNA and greatly improve the utilization rate of effective data;
B. Personalized solutions: In addition to routine analysis, it can provide multiple advanced analysis to provide the best solution for your project.