Customized multiplex immunofluorescence panel_Wuhan biological Optical Valley Science and technology

Login| Register| 中文

搜索



Message

Telephone

027-5981 1720

WeChat

TOP

Current location：

Homepage

/

Technology Services

/

Cell-based immune-related assays

/

Pathology testing services

/

Customized multiplex immunofluorescence panel

技术平台 



Single Cell Sequencing Service 
- Single Cell Sequencing 
- Spatial transcriptome sequencing 
  - Spatial Gene Expression for Fresh Frozen
  - Spatial Gene Expression for FFPE
High throughput sequencing services 
Cell-based immune-related assays 
Proteomics testing service 
- Proteomics services by LC/MS 
- Olink Proteomics Services 
  - Olink Precision Proteomics Analysis Service
Small molecule metabolite detection services 
- Metabolomics service 
  - Targeted metabolomics
  - Non-targeted metabolomics
- Nucleic acid mass spectrometry detection service

Customized multiplex immunofluorescence panel

Product Description

Case Analysis

Result display

Sample delivery

Q&A

Product introduction

Multiplex immunohistochemistry is a tyramine dignal amplification (TSA)-based immunostaining technique that can multiplex multiple molecular markers on a slide. We can provide customized solutions for each project, and quickly achieve quantitative pathology analysis results with up to 9 color multiplex staining through standardized preparation and high-throughput tissue section panoramic quantitative analysis system .We can perform qualitative, conformal and quantitative analysis of tissues, cells and proteins in the images with the help of post-staining antibody elution technology, spectral signal recognition technology and AI intelligent quantitative pathology analysis software. Finally ,the data results are visualized in the form of figures.

Product Procedure

Result Disp

Products and lead-time

Project scope		Services	Services
Multiplex immunofluorescence experiments	Paraffin section	Paraffin section pre-test	3 working days per indicator
	Paraffin section	Paraffin section formal experiment	30 working days
	Tissue microarray	Tissue microarray pre-test	3 working days per indicator
	Tissue microarray	Tissue microarray formal experiment	30 working days
Scanning and analysis service	Scanning film	Multispectral imaging	5 working days
	Analysis project	H-Score scoring	3 working days
		Positive rate	3 working days
		Tissue partitioning	3 working days
		Multi-positive Count	5 working days
		Customized analysis	One project one negotiation

Notices of samples delivery

1. Sample information

(1) Tissue origin: All specimens to be tested should provide accurate information about tissue type (e.g. melanoma, non-small cell lung cancer, colon cancer) and species (e.g. human origin, mouse).

(2) Labeling number: Each slide or wax block should be labeled with a clearly identifiable "unique" number, and TMA slides should be provided with the chip layout and number.

2. Sample types

(1) Formalin-fixed wax blocks or slides, large pieces of tissue or TMA are acceptable.

(2) The sealing wax should not have obvious breakage.

(3) For slide samples, the tissue needs to fit snugly on the slide, avoiding folds, and the slide should not be broken, scratched or stained.

(4) The tissue should contain a minimum of more than 1000 cells.

3. Wax block requirements

(1) The wax block should be embedded with solid tumor tissue; necrotic tumor tissue, needle punctures tissue, and cytospin samples are not acceptable.

(2) Tissue should be fixed with 10% neutral formalin or 4% paraformaldehyde, with a fixation time of 18~24 hours.

(3) Samples fixed by other methods need to be clearly indicated.

(4) The wax block box should have clear and unambiguous number information (please do not just label with a simple number such as 1, 2, 3).

(5) Wax blocks should be stored in a sealed bag with the block number indicated.

4. Slides requirements

(1) The thickness of the slides should be about 3~4um. Please use anti-dislodging slides! (Very important)

(2) It is recommended that the slides should be prepared within one week after fixation.

(3) Prepare at least 2 slides for one sample to be tested.

(4) Do not add any adhesive during water bath.

(5) The sample should be placed in the middle of the slide.

(6) Place the slide upright on absorbent paper to remove water, remove water droplets by tapping slightly. Please do not wipe the slide with paper!

(7) Place the slide on a 45 degree hot plate for 30 min (naturally drying process should not be less than 1 hour).

(8) Slides must be completely dry before transportation.

(9) Each slide must be clearly numbered!

(10) Slides must be placed in a special slide box with the number and inatitution name clearly marked on the box.

(11) If slides have obvious folds and creases.

5. TMA Requirements

(1) Provide a description of the TMA chip point map (several rows and columns).

(2) The TMA slides must be matched with the chip point diagram.

(3) Minimum core point diameter >0.6mm.

(4) TMA core points must not overlap.

(5) Test slides derived from the same TMA wax block (usually incomplete slides from TMA sectioning for pre-experimental testing) need to be provided.

6. Transportation requirements

(1) Samples from different project groups can be transported together, but each group must be placed in a separate package and be clearly marked with: project number (customer order number) \ list of sample numbers \ contact person \ mobile phone number.

(2) Wax block: placed in a separate sealing pocket.

(3) Slides: placed inside the slide box, labeled clearly.

(4) TMA: placed inside the slide box with the instructions of the slide number.

(5) Please use an efficient courier channel such as S.F. to avoid samples being trasnported for a long time.