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Platform introduction

From sample processing to information analysis, Wuhan National Human Genetic Resources Library promises a meticulous service cycle and considerate after-sales experience, a strict and complete production management and service system and a large number of technical teams to provide better services and sequencing programs for scientific research experts in medical, agricultural and other multidisciplinary fields.

Life platform

Life platform

Ion Proton developed by Life Technologies ™  The gene sequencer has passed the CFDA certification in China. It uses a new generation of semiconductor sequencing technology and has excellent performance in compatibility, applicable sample types and multi-target/sample parallel detection. Ion technology is fast, simple and scalable, which can effectively promote the development of clinical research in the diagnosis of cancer and genetic diseases.

Illumina&BGI Platform
Illumina&BGI Platform
Illumina&BGI Platform

Illumina&BGI Platform

Wuhan National Human Genetic Resource Bank currently has MiSeq and NovaSeq6000 high-throughput sequencing platforms and Huada DNBSEQ-T7 sequencing platforms, which have outstanding advantages of high accuracy, high throughput, high sensitivity and low operating costs. It can simultaneously complete traditional genomics research (sequencing and annotation) and functional genomics (gene expression and regulation, gene function, protein/nucleic acid interaction).

PacBio Platform
PacBio Platform

PacBio Platform

The PacBio sequencing platform is based on the single molecule real time (SMRT) technology, through its ultra-long read length, uniform coverage and high consistency. Accurate in-depth analysis of genetic information is provided to create high-quality and high-integrity genome reassembly results for researchers, making many challenging genomics research possible.

Service Items

DNA sequencing services
RNA sequencing services
Epigenetic sequencing service
Macrogenome sequencing
Macrogenomic sequencing aims to sequence the genomes of microbial communities in specific environmental samples in order to analyse the genetic composition and function of microbial communities, to decipher the diversity and abundance of microbial communities, explore the relationship between microbes and their environment, microbes and hosts, and discover and study new genes with specific functions. Macrogenomics is a new approach to study microbial diversity, population structure, evolutionary relationships, functional activity, interactions and relationships with the environment, using the genome of the microbial community in environmental samples as the object of study and functional gene screening and/or sequencing as the means of study. This technique can be used to unravel the diversity and abundance of microbial populations, explore the relationships between microbes and their environment, microbes and their hosts, and discover and study new, functionally specific genes.   technology roadmap：   technical parameter： Sample requirements Sequencing strategy Lead time Sample type: Environmental microbial DNA samples Sample concentration: ≥50 ng/μl Total sample mass: ≥3 μg Sequencing mode: Illumina/MGI/Double-end sequencing Sequencing depth: 5-10G clean data 45 days   Product advantages. a. Technical advantages: PCR-free library construction for more accurate species annotation, and new splicing methods to reduce the false positive rate of gene annotation; b. Customised solutions: In addition to conventional analyses, a number of advanced analyses are available to provide the best solution for your project.

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Whole genome sequencing
Whole Genome Sequencing (WGS) is a genome-wide sequencing method that can decipher all common and rare variants, single nucleotide polymorphisms (SNP), insertion and deletion sites (InDel), structural variants (SV) and copy number variants (CNV), and thus reveal all types of genomic mutations and chromosomal rearrangement events. This will provide guidance for disease mechanism research and drug target screening. Applications include clinical medicine, population genetics, association analysis, evolutionary analysis and so on.   technology roadmap   technical parameter   Technical Parameters Sequencing strategy Lead time Sample type: Genomic DNA Sample concentration: ≥20 ng/μl (Qubit quantification) Total sample mass: ≥3.0 μg (total of 3 library builds, excluding consuming of sample assay, Qubit quantification) Sample purity:  OD260/280 =1.7~2.2 Electrophoresis requirements: clear primary bands, no degradation or light degradation, no serious RNA or protein contamination (latest electrophoresis results of the sample delivery date).   Sequencing mode: Illumina/MGI Sequencing depth: 30X recommended 45 days   Product advantages a. High accuracy: Using the Illumina /MGI platform by which more than a million sequences can be performed in a single run, with accurate counts ranging from several to hundreds of thousands copies; b. Wide detection range: single nucleotide polymorphism (SNP), insertion deletion (InDel), structural variation (SV), copy number variation (CNV) and other variants can be detected, and new variant sequences can be detected compared to microarrays; c. Low cost and less time consuming than whole human genome de novo sequencing.

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Exome sequencing
Whole Exome Sequencing (WES) refers to the use of sequence capture technology to capture and enrich DNA from exome regions for high-throughput sequencing, which can directly identify genetic variants associated with protein function variation. Exome sequencing has been applied to the search for disease-causing and susceptibility genes associated with a variety of complex diseases.                technology roadmap     technical parameter   Sample requirements Sequencing strategy Lead time Sample type: genomic DNA Total sample concentration: ≥20 ng/μl (Qubit quantification) Total sample mass: ≥3.0 μg (total of 3 times of library building, excluding consuming of sample assay, Qubit quantification) Sample purity: OD260/280 =1.7~2.2 Electrophoresis requirements: clear main band, no degradation or mild degradation, no serious RNA or protein contamination. (latest electrophoresis result of the sample delivery date)   Sequencing type: Illumina/MGI PE150. Sequencing depth: germ cell mutations ≥ 100X (10G). Somatic mutations ≥150X (15G)  45 days       Product advantages a. High specificity: Use mainstream kit, have stable capture efficiency, comprehensive exon region coverage and high capture specificity; b. Cost-effective: comprehensive functional annotation of exon regions, which can effectively identify disease- or trait-related regions and save research costs; c. High information efficiency: Compared with whole genome sequencing, it has deeper coverage and better data precision, less interference information and easy to analyse.

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Macro-transcriptome sequencing
Macro-transcriptomics studies the genome-wide transcription of organisms and the regulation of transcription at a holistic level for a specific environment and a specific period of time in a community. Macro-transcriptome sequencing can study in situ the composition of active strains and the expression of active genes in a microbial community of the specific habitat at a specific time and space. In combination with the detection of physicochemical factors, macro-transcriptome can study the differences in the composition of active components between microbial communities in space and time due to the differences in physicochemical and other indicators among the samples. Technical route:     　　Sample requirements： Sample type Sequencing strategy Lead time Sample concentration: ≥100 ng/μl Total sample  mass: ≥4 μg Sample purity: OD260/280=1.8~2.2, OD260/230=1.8~2.2; 28S: 18S≥1. Integrity:RIN≥7 Sequencing mode: Illumina/MGI PE150 Sequencing depth: 6-12G clean data 45 days   Product advantages: A. Technical advantages: the official ribosome removal kit can effectively remove rRNA and greatly improve the utilization rate of effective data; B. Personalized solutions: In addition to routine analysis, it can provide multiple advanced analysis to provide the best solution for your project.

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Long-strand non-coding RNA sequencing
lncRNAs are greater than 200bp in length and do not encode proteins, which are widely found in living organisms. lncRNAs regulate gene expression levels at multiple levels (epigenetic regulation, transcriptional regulation, and post-transcriptional regulation, etc.) and play an important role in life activities. lncRNA sequencing is a research method that uses a specific method to reduce the abundance of rRNAs in samples, during which a library to sequence the enriched lncRNA sequencing would be constructed and almost all the lncRNA information can be obtained at one time,  providing a comprehensive and in-depth analysis of lncRNA classes and functions to get lncRNA related information of specific biological processes (e.g. development, diseases, etc.) quickly and accurately.   Technical route:   technical parameter：   Sample type Sequencing strategy Lead time Sample type: Total RNA Sample concentration: ≥100 ng/μl Total sample mass: ≥5 μg Sample purity:OD260/280=1.8~2.2, OD260/230 = 1.8~2.2 Integrity:RIN≥7 Sequencing mode: Illumina/MGI PE150 Sequencing depth: 10G /12G clean data 45 days   Product advantages. a. Cost-effective: one library can obtain both mRNA and lncRNA information; b.Total solution: one-stop upstream sequencing & downstream functional validation and consulting services; c. Comprehensive services: standard analysis, advanced analysis and multi-omics association analysis are all available.  

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Macro-transcriptome sequencing
Macro-transcriptomics studies the genome-wide transcription of organisms and the regulation of transcription at a holistic level for a specific environment and a specific period of time in a community. Macro-transcriptome sequencing can study in situ the composition of active strains and the expression of active genes in a microbial community of the specific habitat at a specific time and space. In combination with the detection of physicochemical factors, macro-transcriptome can study the differences in the composition of active components between microbial communities in space and time due to the differences in physicochemical and other indicators among the samples.   　　technology roadmap：   　　Reference technology：   Sample requirements Sequencing strategy Lead time Sample type: Environmental microbial RNA samples Sample concentration: ≥100 ng/μl Total sample  mass: ≥4 μg Sample purity: OD260/280=1.8~2.2, OD260/230=1.8~2.2; 28S: 18S≥1. Integrity:RIN≥7 Sequencing mode: Illumina/MGI PE150 Sequencing depth: 6-12G clean data 45 days   　Product advantages: A. High coverage Through different sequencing schemes, almost all the transcriptome expression and structure information of samples can be obtained, and the transcriptome information of low abundance can be obtained at the same time; B. Multi-platform solutions: provide Illumina and Pacbio multi-platform solutions; C. No species restriction: the detection scope covers all microorganisms, animals and plants and human samples.

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