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Progenitor cell/3D organoid culture

Platform Introduction

The cell platform mainly provides services related to the detection of cellular immune function. At present, the number of samples assisted in analysis is close to one million, and it has accumulated rich experience in sample pretreatment, detection and analysis.
The platform technicians mainly carry out systematic basic medicine and clinical research on malignant tumors, inflammation and autoimmune diseases with the background of biology, basic medicine, immunology and other related disciplines. The research direction covers disease pathogenesis research, new vaccine research and development and evaluation, new anti-tumor drug screening, effectiveness and safety evaluation, drug action mechanism research and other aspects. Recently, a primary tumor cell and organ-like bank is being built for fresh cancer and paracancerous tissue samples from cancer patients.

 

Service Items

Multiple cytokine expression assessment
Cytokines, as intercellular signaling molecules, mainly mediate and regulate the immune response and inflammatory reaction, and maintain the stability of the body's immune system by regulating the dynamic balance between immune promotion and immune suppression. The detection of cytokines is of great value for the diagnosis and treatment of certain diseases. Extracellular (serum, cell culture fluid, lavage fluid, etc.) cytokines reflect the level of secretion.     Instrument used: Cytoflex S (4 laser 13 colors), Cytoflex LX (6 laser 21 colors)    Application areas: immunology, hematology, oncology, bone marrow and organ transplantation, antitumor drug research, vaccine research, basic medical research, etc. Project advantages Flow cytometry extracellular factor assay (superior to Elisa, more than 10 cytokines simultaneously in 5 hours can be detected with only 25 μL sample, which greatly saves the amount of sample, time cost and experimental cost, and has high sensitivity and specificity) Lead time: 项目类型 项目内容 服务周期 Detection of secretory cytokine concentrations in body fluids Th1/Th2/Th9/Th17/Th22/anti-inflammatory, pro-inflammatory cytokines (IL-2, IL-6, IL-10, IFN-γ, TNF-α, IL-5, IL-13, IL-4, IL-17A, IL-17F, IL-22, IL-9, CXCL8 (IL-8), CXCL10 (IP-10), CCL11 (Eotaxin), CCL17 (TARC), CCL2 (MCP-1), CCL5 (RANTES), CCL3 (MIP-1α), CXCL9 (MIG), CXCL5 (ENA-78), CCL20 (MIP-3α), CXCL1 (GROα), CXCL11 (I-TAC), etc. ) 1-3 working days Note: The above lead time is under the condition of reagents provided by Party A( the client ). If Party B (the company )'s previous reagents have been ran out ,another 4-5 weeks is needed in addition for purches related reagents .
Ready-to-use multiplex immunofluorescence panel
Product introduction This product is used for tumor immune microenvironment assessment and clinical development and translational research of tumor immune drugs, covering multiple dimensions such as tumor immunotherapy targets and tumor immune cells. Through high specificity multiplex fluorescence staining, spectral imaging and high throughput image analysis technologies, we can accurately capture tumor microenvironment data to empower anti-tumor drug development.     Sample image of product BB02     Service products and cycle Product Number     Indicators Tissue Phenotype Data Analysis Lead time BB01 CD3、CD20、CD68、 CD163、Ki67、pan-CK、DAPI T cells, B cells, M1 macrophages, M2 macrophages Standard analysis. 1. tissue partitioning: tumor and interstitial. 2. cell typing: defining the different cell types separately and calculating the number, density and percentage of cells. Extended analysis. 1. Evaluation of expression indicators TPS and CPS; 2  statistics on the number, density, and percentage of immune checkpoint molecules expressed in different types of cells. 3. spatial analysis.   10 working days BB02 CD4、CD8、FOXP3、 PD-1、PD-L1、pan-CK、DAPI Helper T cells, killer T cells BB03 CD8、CD68、CD56、 PD-1、PD-L1、pan-CK、DAPI Treg, immune checkpoint killer T cells, total macrophages, Depleted T cells, NK cells, immune checkpoints BB04 PD-1、PD-L1、CD68、 CD8、CD163、pan-CK、DAPI M1 macrophages, M2 macrophages, killer T cells, immune checkpoints BB05 CD3、CD4、CD20、 CD56、FOXP3、pan-CK、DAPI depleted T cells, immune checkpoints total T cells, helper T cells. B cells, NK cells, Treg BB06 CD4、CD8、GZMB、 Ki67、FOXP3、pan-CK、DAPI depleted T cells, immune checkpoints total T cells, helper T cells. B cells, NK cells, Treg BB07 CD11c、CD20、CD68、 CD163、CD3、pan-CK、DAPI tertiary lymphoid structures, B cells, T cells, and Germinal center activated B cells, follicular dendritic cells Tertiary lymphoid structures, B cells, killer T cells BB08 CD20、CD21、CD23、 CD3、Ki67、pan-CK、DAPI tertiary lymphoid structures, B cells, T cells, and Germinal center activated B cells, follicular dendritic cells BB09 CD20、CD23、CD8、 PD-1、PD-L1、pan-CK、DAPI Tertiary lymphoid structures, B cells, killer T cells follicular dendritic cells, depleted T cells, immune checkpoints BB10 PD-1、CD8、CD68、 CD56、FOXP3、pan-CK、DAPI killer T cells, total macrophages, NK cells, Treg, immune checkpoints  

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