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Cell-based immune-related assays
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Flow cytometry-based assays

Platform Introduction

The cell platform mainly provides services related to the detection of cellular immune function. At present, the number of samples assisted in analysis is close to one million, and it has accumulated rich experience in sample pretreatment, detection and analysis.
The platform technicians mainly carry out systematic basic medicine and clinical research on malignant tumors, inflammation and autoimmune diseases with the background of biology, basic medicine, immunology and other related disciplines. The research direction covers disease pathogenesis research, new vaccine research and development and evaluation, new anti-tumor drug screening, effectiveness and safety evaluation, drug action mechanism research and other aspects. Recently, a primary tumor cell and organ-like bank is being built for fresh cancer and paracancerous tissue samples from cancer patients.

 

Service Items

Flow cytometry
Multiple immunofluorescence detection technology
Primary cell/type 3D organ culture
Patch clamp
Immune cell typing and functional assessment
Flow cytometry (FCM) is a multi-parameter, rapid quantitative analysis and sorting technique for cells or particles in a fast, linear and stable flow state using a laser as the light source. In addition to conventional apoptosis and cycling, it can be applied to immune cell typing, intracellular/extracellular cytokine and phosphorylated protein detection, stem cell phenotype identification, plant ploidy/genome size assessment, and high-throughput single cell sorting for downstream sequencing.

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Multiple cytokine expression assessment
Cytokines, as intercellular signaling molecules, mainly mediate and regulate the immune response and inflammatory reaction, and maintain the stability of the body's immune system by regulating the dynamic balance between immune promotion and immune suppression. The detection of cytokines is of great value for the diagnosis and treatment of certain diseases. Extracellular (serum, cell culture fluid, lavage fluid, etc.) cytokines reflect the level of secretion.     Instrument used: Cytoflex S (4 laser 13 colors), Cytoflex LX (6 laser 21 colors)    Application areas: immunology, hematology, oncology, bone marrow and organ transplantation, antitumor drug research, vaccine research, basic medical research, etc. Project advantages Flow cytometry extracellular factor assay (superior to Elisa, more than 10 cytokines simultaneously in 5 hours can be detected with only 25 μL sample, which greatly saves the amount of sample, time cost and experimental cost, and has high sensitivity and specificity) Lead time： 项目类型 项目内容 服务周期 Detection of secretory cytokine concentrations in body fluids Th1/Th2/Th9/Th17/Th22/anti-inflammatory, pro-inflammatory cytokines (IL-2, IL-6, IL-10, IFN-γ, TNF-α, IL-5, IL-13, IL-4, IL-17A, IL-17F, IL-22, IL-9, CXCL8 (IL-8), CXCL10 (IP-10), CCL11 (Eotaxin), CCL17 (TARC), CCL2 (MCP-1), CCL5 (RANTES), CCL3 (MIP-1α), CXCL9 (MIG), CXCL5 (ENA-78), CCL20 (MIP-3α), CXCL1 (GROα), CXCL11 (I-TAC), etc. ) 1-3 working days Note: The above lead time is under the condition of reagents provided by Party A( the client ). If Party B (the company )'s previous reagents have been ran out ,another 4-5 weeks is needed in addition for purches related reagents .

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High throughput cell sorting
Flow cytometry sorting is based on flow cytometry analysis, which can "pick out" the target cells of interest as the material for subsequent experimental operations such as culture amplification, protein identification, and sequencing. The challenge for sorting is to obtain cells with high viability, purity and recovery in a fast and accurate way.     Instrument used: Moflo XDP (3 laser 10 colors) Application areas: immunology, hematology, oncology, bone marrow and organ transplantation, antitumor drug research, vaccine research, basic medical research, etc. Project advantages. In the case of cellular raw material acquisition based on single cell sequencing, SMART Seq2, RNA Seq and other sequencing, flow cytometry sorting is the best choice for efficient screening of high viability and high purity target cells. Through genome sequencing, single cell sequencing, proteomics and bioconfidence platform, we can achieve one-stop service from raw material to report issuance. Lead time： Project type  Project Content Service period High throughput cell flow cytometry sorting   Stable transfer cell line screening      1-3 working days Antigen-Specific B-cell Screening    1-3 working days 96-1536 well plate single cell sorting 1-3 working days Multi-color, four-way sorting 1-3 working days Sorting of target cells for downstream sequencing, single cell sequencing, proteomics, etc.     1-3 working days  

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Flow cytometry special service
Extracellular vesicles (EV) are considered as potential non-invasive biomarkers in disease diagnosis and mediators in drug delivery with a high degree of heterogeneity. Transcriptome studies of single EVs are valuable to better define EV characteristics, EV subgroups and clinical applications. For EV identification and concentration assessment, it is a critical step for single cell sequencing to be successfully brought online. The use of flow cytometry-based means enables the size-specific localization of EVs, thus allowing the enumeration of integrity vesicles as well as the adjustment of appropriate concentrations for subsequent single-cell sequencing.

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Customized multiplex immunofluorescence panel
Multiplex immunohistochemistry is a tyramine dignal amplification (TSA)-based immunostaining technique that can multiplex multiple molecular markers on a slide. We can provide customized solutions for each project, and quickly achieve quantitative pathology analysis results with up to 9 color multiplex staining  through standardized preparation and high-throughput tissue section panoramic quantitative analysis system .We can perform qualitative, conformal and quantitative analysis of tissues, cells and proteins in the images with the help of post-staining antibody elution technology, spectral signal recognition technology and AI intelligent quantitative pathology analysis software. Finally ,the data results are visualized in the form of figures.

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Ready-to-use multiplex immunofluorescence panel
Product introduction This product is used for tumor immune microenvironment assessment and clinical development and translational research of tumor immune drugs, covering multiple dimensions such as tumor immunotherapy targets and tumor immune cells. Through high specificity multiplex fluorescence staining, spectral imaging and high throughput image analysis technologies, we can accurately capture tumor microenvironment data to empower anti-tumor drug development.     Sample image of product BB02     Service products and cycle Product Number     Indicators Tissue Phenotype Data Analysis Lead time BB01 CD3、CD20、CD68、 CD163、Ki67、pan-CK、DAPI T cells, B cells, M1 macrophages, M2 macrophages Standard analysis. 1. tissue partitioning: tumor and interstitial. 2. cell typing: defining the different cell types separately and calculating the number, density and percentage of cells. Extended analysis. 1. Evaluation of expression indicators TPS and CPS; 2  statistics on the number, density, and percentage of immune checkpoint molecules expressed in different types of cells. 3. spatial analysis.   10 working days BB02 CD4、CD8、FOXP3、 PD-1、PD-L1、pan-CK、DAPI Helper T cells, killer T cells BB03 CD8、CD68、CD56、 PD-1、PD-L1、pan-CK、DAPI Treg, immune checkpoint killer T cells, total macrophages, Depleted T cells, NK cells, immune checkpoints BB04 PD-1、PD-L1、CD68、 CD8、CD163、pan-CK、DAPI M1 macrophages, M2 macrophages, killer T cells, immune checkpoints BB05 CD3、CD4、CD20、 CD56、FOXP3、pan-CK、DAPI depleted T cells, immune checkpoints total T cells, helper T cells. B cells, NK cells, Treg BB06 CD4、CD8、GZMB、 Ki67、FOXP3、pan-CK、DAPI depleted T cells, immune checkpoints total T cells, helper T cells. B cells, NK cells, Treg BB07 CD11c、CD20、CD68、 CD163、CD3、pan-CK、DAPI tertiary lymphoid structures, B cells, T cells, and Germinal center activated B cells, follicular dendritic cells Tertiary lymphoid structures, B cells, killer T cells BB08 CD20、CD21、CD23、 CD3、Ki67、pan-CK、DAPI tertiary lymphoid structures, B cells, T cells, and Germinal center activated B cells, follicular dendritic cells BB09 CD20、CD23、CD8、 PD-1、PD-L1、pan-CK、DAPI Tertiary lymphoid structures, B cells, killer T cells follicular dendritic cells, depleted T cells, immune checkpoints BB10 PD-1、CD8、CD68、 CD56、FOXP3、pan-CK、DAPI killer T cells, total macrophages, NK cells, Treg, immune checkpoints  

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Multi-color pathology data analysis
Product description Using spectral signal recognition technology, the overlapping multi-color signals could be accurately split, and with inForm intelligent quantitative pathology analysis software and professional bioinformatics analysis team, qualitative, conformal and quantitative analysis of tissues, cells and proteins in the images could be performed, and finally the data results are visually displayed in the form of visual figures, providing customers with standardized and customized data analysis to meet different types of needs.

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