Organoid Authentication-Wuhan Biobank Co., Ltd.

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Single Cell Sequencing Service
- Single Cell Sequencing
- Spatial transcriptome sequencing
  - Spatial Gene Expression for Fresh Frozen
  - Spatial Gene Expression for FFPE
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Cell-based immune-related assays
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- Proteomics services by LC/MS
- Olink Proteomics Services
  - Olink Precision Proteomics Analysis Service
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- Metabolomics Services
  - Targeted metabolomics
  - Non-targeted metabolomics
- Nucleic acid mass spectrometry detection service

Organoid Authentication

Product Description

Case Analysis

Results Display

Sample Submission Guidelines

Q&A

Authentication Process:

1.Histopathological Morphological Analysis (H&E Staining): Assesses structural similarity between organoids and source tissue.

2.Genetic Lineage Tracing (Sequencing): Evaluates genetic correlation and determines purity via copy number variation (CNV) analysis.

3.Surface Marker Detection (Flow Cytometry, mIHC): Confirms expression of specific protein markers.

4.Functional Assessment: Viability and proliferation capacity testing.

Authentication Case Studies:

Figure: Comparison of intestinal organoids with intestinal tissue (H&E staining): Morphological pathological analysis via H&E staining indicates structural similarity between constructed organoids and source tissue.

Figure: Immunofluorescence identification of colorectal cancer organoids: Multi-color immunofluorescence identifies expression of CAM5.2, HER2, CLDN18.2, FGFR, and CEA.

Figure: Flow cytometric analysis of epithelial malignancy marker EpCAM in colorectal cancer organoids: 98.7% of cells express EpCAM.

Figure: Genetic analysis reveals high correlation between colorectal cancer organoids and corresponding tissue based on germline and somatic mutations, confirming shared individual origin.