Organoid Authentication
Authentication Process:
1.Histopathological Morphological Analysis (H&E Staining): Assesses structural similarity between organoids and source tissue.
2.Genetic Lineage Tracing (Sequencing): Evaluates genetic correlation and determines purity via copy number variation (CNV) analysis.
3.Surface Marker Detection (Flow Cytometry, mIHC): Confirms expression of specific protein markers.
4.Functional Assessment: Viability and proliferation capacity testing.
Authentication Case Studies:
Figure: Comparison of intestinal organoids with intestinal tissue (H&E staining): Morphological pathological analysis via H&E staining indicates structural similarity between constructed organoids and source tissue.
Figure: Immunofluorescence identification of colorectal cancer organoids: Multi-color immunofluorescence identifies expression of CAM5.2, HER2, CLDN18.2, FGFR, and CEA.
Figure: Flow cytometric analysis of epithelial malignancy marker EpCAM in colorectal cancer organoids: 98.7% of cells express EpCAM.
Figure: Genetic analysis reveals high correlation between colorectal cancer organoids and corresponding tissue based on germline and somatic mutations, confirming shared individual origin.
